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Jaffe test (for creatinine) to the liquid add trinitrophenol and then make alkaline with sodium hydroxide. A red color indicates presence of creatinine. (for indican) to the suspected liquid are added an equal amount of concentrated hydrochloric acid, 1 mL of chloroform, and a few drops of a strong solution of chlorinated soda. The chloroform is colored blue if indican is present.
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