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blot (blot) to transfer ionic solutes, such as DNA, RNA, or proteins, onto a membrane, filter, treated paper, or other immobilizing matrix for analysis, often after first using electrophoresis to separate fragments of different size. the matrix containing the transferred material in such a process. dot blot
a technique used to detect and analyze nucleic acids or proteins, in which samples are spotted directly onto the substrate,
without first using electrophoresis to separate them. Northern blot
a technique analogous to a Southern blot but performed on fragments of RNA. Southern blot
a technique (named for E.M. Southern, the scientist who invented it) in which DNA fragments are separated by electrophoresis,
transferred onto an immobilizing membrane, and analyzed. Western blot
a technique analogous to a Southern blot but performed on protein fragments.
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