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antimetabolite (an″te-) (an″ti-mә-tabĀ“o-līt) a substance bearing a close structural resemblance to one required for normal physiological functioning, and exerting its effect by interfering with the body's use of the essential metabolite. a class of antineoplastic agents consisting of antimetabolites of substances required for cell growth and replication; the interference with cell function is phase specific, largely in the S phase of the cell cycle. See also antineoplastic therapy.
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